It is really necessary to have an internal standard for every metabolite measured for appropriate and reproducible metabolic profiling. IROA internal standard kits and IROA quantitation kits are the equipment that you can use for metabolic profiling. With IROA quantitation kits, you will be able to make your own internal standards that are mirroring the metabolome to be measured. These kits include all of the reagents that are needed for labeling, quantitating, and identifying biochemical elements in cell populations fruitfully
With IROA 100, you can effectively create yeast internal standards, also can identify and quantitate yeast metabolites. With IROA 200, you will be able to create bacterial internal standards, identify and quantitate bacterial metabolites. And with IROA 300, you will be capable of creating mammalian internal standards, identify and quantitate mammalian metabolites. Mammalian metabolic profiling kits comprises 13C metabolite yeast extract.
Then again, IROA internal standard kits contain complicated internal standards that are pre-labeled. It can be used for the identification of hundreds of metabolites across time and tools. You will get Cluster Finder software and statistical analysis tools with buying of any kits.
In the following passages, you will get to know about the IROA metabolic profiling tools that contain 5% and 95% uniformly 13C-labeled yeast extract. Take a look at the adjoined passages of this article appropriately for knowing more about this topic
Know about IROA metabolic profiling tools containing 13C metabolite yeast extract
IROA has introduced two quantitation kits for exact mammalian metabolic profiling. This part of these articles contains some significant factors you require knowing about these kits. Read the below passages carefully to know about them fully.
IROA Fluxomic Quantitation Kit
This kit has two components: Labeling Medium, which is liquid and Component Mix, which is dry. The labeling medium includes 1 bottle of 250 mL EBSS/RPMI 1640 vitamin solution having 1mg/mL 5% U-13C labeled glucose and 1 empty sterile bottle. You can store media following filter sterilization in it. The component mix comprises 657.5mg 5% U-13C metabolite yeast extract and amino acids.
This kit provides the reagents to fully label mammalian cells at 5% U-13C. You may use this kit in order to decide the flux of a definite precusor through metabolic pathways. The use of 5% will help you in identifying all metabolic tools. This tool also helps in eliminating non-biological signals in an entirely unbiased and non-targeted manner.
IROA Phenotypic Quantitation Kit
This quality IROA kit for mammalian metabolic profiling also includes labeling medium and element mix. The labeling medium contains 1 bottle of 250 mL EBSS/RPMI 1640 vitamin solution including 1mg/mL 95% U-13C labeled glucose and 1 empty sterile bottle. The component mix is 657.5mg 95% U-13C labeled yeast extract and amino acids.
This effective kit is designed for situations where it is impossible to label experimental samples in cell culture systems like biopsies, field-grown plants, or large-scale fermentation.
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To find more information about IROA metabolic profiling tools and 13C metabolite yeast extract metabolic standard, you can contact “IROA Technologies LLC”. Log on to iroatech.com to know more.